Inhibition of Alanine and Aspartate Aminotransferases by B-Nitropropionic Acid

Comparison of specific and relative alanine and aspartate aminotransferases of Trypanosoma brucei subgroup trypanosomes.

Using Biochemica Test Kits (Boehringer GmbH, Mannheim) a significant difference in alanine aminotransferase : aspartate aminotransferase (ALAT : ASAT) ratios was found between Trypanosoma brucei brucei (4 strains; mean ± S.E.; 4.204 ± 0.342) and T. b. rhodesiense (4 strains; mean ± S.E.: 2.973 ± 0.174) blood¬ stream stage crude soluble enzyme extracts. This difference is a stable characteristic...

Effect of cortisone on aspartate and alanine aminotransferases in a desert lizard.

1. Liver and serum aspartate aminotransferase (GOT) and alanine aminotransferase (GPT) activities were measured in a hibernating desert lizard, Uromastix hardwickii. The levels of both enzymes were found to be lower in hibernation than during the active period, particularly in the liver. 2. After intramuscular injection of 2 mg of cortisone acetate there was a rapid rise in the levels of these ...

Primary structures of aspartate aminotransferases.

three residues seems to be common to all the serine enzymes and has become known as the ‘charge-relay system’. Its function is to transfer a negative (or partial negative) charge from the aspartic acid residue to the oxygen atom of the hydroxy group of serine. This obviously increases the nucleophilicity of the serine, but the important point is that the charge is developed in the absence of bu...

Quantitative Analysis of Serum Level Alanine and Aspartate Aminotransferases, γ-Glutamyl Transferase and Alkaline Phospatase as Predictor of Liver Diseases

3-Nitropropionic acid toxicity in hippocampus: protection through N-methyl-D-aspartate receptor antagonism.

The over-activation of glutamate receptors can lead to excitotoxic cell death and is believed to be involved in the progression of neurodegenerative events in the vulnerable hippocampus. Here, we used an in vitro slice model to study toxicity produced in the hippocampus by the mitochondrial toxin 3-nitropropionic acid (3-NP). The organotypic slice cultures exhibit native cellular organization a...